Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225348_n5kv GCTACTTAAAATGTAGACTGTTCATACAGTGG(+CAGTGT)GTACCAGTATGAGTTGAATGTGTGTATTACT NGG 10 8 17 8 24 0 100 5 10
    240605225346_6oxr CGATTCTCCATATCCATTCTTCTCCATAGAAG(+CAGTGT)GTACCCTAAGCTAAAGAAGATACCGCTATGC NGG 10 8 17 8 24 0 100 5 10
    240605225345_3qgv TCCAGGCATAGCGGTATCTTCTTTAGCTTAGG(+CAGTGT)GTACCTTCTATGGAGAAGAATGGATATGGAG NGG 10 8 17 8 24 0 100 5 10
    240605225343_5jlu TAAATAAAGAAAATATCTAATAAAAAAAAAAG(+CAGTGT)GATCCTTCTATTCTAGTTATCTTTTCCACAA NGG 10 8 17 8 24 0 100 5 10
    240605225342_aed8 TTTTATTGTGGAAAAGATAACTAGAATAGAAG(+CAGTGT)GATCCTTTTTTTTTTATTAGATATTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605225340_n7fq CAGAGGAATCCTTCAAGCTTCAGTCCTGCCTG(+CAGTGT)GATCCTGCTTCATCTTGCCTCCCTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605225339_dsa1 ATCAGTCCAGCCAGGGAGGCAAGATGAAGCAG(+CAGTGT)GATCCAGGCAGGACTGAAGCTTGAAGGATTC NGG 10 8 17 8 24 0 100 5 10
    240605225338_k27j ACAAATGAGTCCAGGAAGATGGAGCCCTTTAG(+CAGTGT)GATCCATCAAGCCCCAAATGAAAGCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240605225336_g8ma AATAAAAGCAAGCTTTCATTTGGGGCTTGATG(+CAGTGT)GATCCTAAAGGGCTCCATCTTCCTGGACTCA NGG 10 8 17 8 24 0 100 5 10
    240605225334_3duv TTCCATTGGGGTGGCCCTACTCCGGCCCTAAG(+CAGTGT)GATCCCCAAGGCACAGCTAATGGTTCCTTAC NGG 10 8 17 8 24 0 100 5 10