Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225707_hcah GGTCTTTTGAGAGATTTATTGAATAAGAACAG(+CAGTGT)GTACCAGCGGGACACTGAAACAGTTATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605225706_z0ue GCACACAGCATAACTGTTTCAGTGTCCCGCTG(+CAGTGT)GTACCTGTTCTTATTCAATAAATCTCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605225704_vvu2 ATGGATATTATATCAGACCTCCTTCTGGTCAG(+CAGTGT)GATCCAGTCTACCACACTTATGACTAAGCGA NGG 10 8 17 8 24 0 100 5 10
    240605225702_fqsh TGATATCGCTTAGTCATAAGTGTGGTAGACTG(+CAGTGT)GATCCTGACCAGAAGGAGGTCTGATATAATA NGG 10 8 17 8 24 0 100 5 10
    240605225701_pcs9 TCAGGGTGTTATCCTCTTCCTGAGACACTCAG(+CAGTGT)GATCCTTGGAAGAACAACTTTTGCTGTCCGT NGG 10 8 17 8 24 0 100 5 10
    240605225659_y0pc GATCCACGGACAGCAAAAGTTGTTCTTCCAAG(+CAGTGT)GATCCTGAGTGTCTCAGGAAGAGGATAACAC NGG 10 8 17 8 24 0 100 5 10
    240605225657_h48j GTTTCTTTCCGAGCTGATGACAAGACTGAATG(+CAGTGT)GTACCTTTTCTTTCTGATCGGTTCTAAACAT NGG 10 8 17 8 24 0 100 5 10
    240605225656_0u5x GGCCAATGTTTAGAACCGATCAGAAAGAAAAG(+CAGTGT)GTACCATTCAGTCTTGTCATCAGCTCGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605225654_kblv CCCCAAGACTTTGTTTTATCGGAGAGGAAAAG(+CAGTGT)GTACCCCATTTCATTCATTGGTGTAAGGATG NGG 10 8 17 8 24 0 100 5 10
    240605225653_93qa GAAAACATCCTTACACCAATGAATGAAATGGG(+CAGTGT)GTACCTTTTCCTCTCCGATAAAACAAAGTCT NGG 10 8 17 8 24 0 100 5 10