Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225206_r2iu CAGATTCTCAGGACATGGTCAGAATGAAGCCG(+CAGTGT)GATCCTTTTTCCAAATAACACATAAATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605225205_9ifn GGCCAGAGGATAACGTTAGAGTTACCAACGTG(+CAGTGT)GATCCTGGGAACCAAACTTAGGTCCTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605225202_tgxm TACTCTTTAGAGGACCTAAGTTTGGTTCCCAG(+CAGTGT)GATCCACGTTGGTAACTCTAACGTTATCCTC NGG 10 8 17 8 24 0 100 5 10
    240605225201_2lwd AGTGTTGGACTCTGTTTTCTGCAATACAAAGG(+CAGTGT)GTACCACAGCAAACAGAAAGGCCAGCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605225159_j4z0 GTCTTTCCTGGCTGGCCTTTCTGTTTGCTGTG(+CAGTGT)GTACCCTTTGTATTGCAGAAAACAGAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605225158_364s GCAGTGCTGGTGGAGGGTAGAAAAAGTTGCTG(+CAGTGT)GATCCTCTGGAGCTGAAGTTACAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605225156_w60h CTTCACAACCATCTGTAACTTCAGCTCCAGAG(+CAGTGT)GATCCAGCAACTTTTTCTACCCTCCACCAGC NGG 10 8 17 8 24 0 100 5 10
    240605225155_40xv TACTGAATTTTTGTCTCATTCACATATTCCAG(+CAGTGT)GTACCAACCACTGTGTGTGACCTACTAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605225153_tui3 TAGTTTCTTTAGTAGGTCACACACAGTGGTTG(+CAGTGT)GTACCTGGAATATGTGAATGAGACAAAAATT NGG 10 8 17 8 24 0 100 5 10
    240605225151_h6l2 GCTGGGAGGGCCGTGTACAGCCCTTGGTCTGG(+CAGTGT)GATCCATGTGTTGGTTGAGAAATCATTCCAG NGG 10 8 17 8 24 0 100 5 10