Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225116_nj20 CGCCTGGAGCCTGGCTGTGTAGGGCAGGGGAG(+CAGTGT)GATCCCTGATGGCAGCTTCCCAGTTGCATAC NGG 10 8 17 8 24 0 100 5 10
    240605225115_3j1b CCTAGAGGCCCTTCCCCGCTGGCGGGTTCATG(+CAGTGT)GATCCTTCTAATAAACTTATGTGTCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605225114_gbr0 GGAAGGAGGAGGACACATAAGTTTATTAGAAG(+CAGTGT)GATCCATGAACCCGCCAGCGGGGAAGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605225112_sx6n GCCCAGAGCTTGTTCTAAACTGCGAAGTCCAG(+CAGTGT)GTACCTAGCCCCACACCTGCTCCTCCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605225111_mudb ACCCCAGGAAGGAGGAGCAGGTGTGGGGCTAG(+CAGTGT)GTACCTGGACTTCGCAGTTTAGAACAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605225109_vxuc TCTGGGAAGACTGGGCTAGAGGAAAGAGACTG(+CAGTGT)GTACCAGTCTTGGCAGGAGGGTGGGGCAAGG NGG 10 8 17 8 24 0 100 5 10
    240605225108_sf02 CAGGACCTTGCCCCACCCTCCTGCCAAGACTG(+CAGTGT)GTACCAGTCTCTTTCCTCTAGCCCAGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605225107_0saj TCTATGGGCATTGGCCCTGACCTAGGAGTCAG(+CAGTGT)GATCCCAGGTCACACCTATGGGTGCTGGCTA NGG 10 8 17 8 24 0 100 5 10
    240605225104_d5wp GTCACTAGCCAGCACCCATAGGTGTGACCTGG(+CAGTGT)GATCCTGACTCCTAGGTCAGGGCCAATGCCC NGG 10 8 17 8 24 0 100 5 10
    240605225103_nfme ACTGAGGTAGGCAGGAGGGCTGGGGTGCTTGG(+CAGTGT)GTACCCCATAATCAGCACAGATGAGGTTGGA NGG 10 8 17 8 24 0 100 5 10