Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225150_ahyb ACACTCTGGAATGATTTCTCAACCAACACATG(+CAGTGT)GATCCCAGACCAAGGGCTGTACACGGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605225148_czrs TGTCCCCTCCCTGGTCTTATGCCACTTGCCAG(+CAGTGT)GTACCCTCAGAGCTGTGGGGTGCCTTAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605225147_lknu ACGCAGAACTAAGGCACCCCACAGCTCTGAGG(+CAGTGT)GTACCTGGCAAGTGGCATAAGACCAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605225145_61b7 AGGCTTAGAACTTGAACTCGCCCTGACCTCTG(+CAGTGT)GATCCTGTTCTTCAAGGAAGCAAAACCAAAT NGG 10 8 17 8 24 0 100 5 10
    240605225143_wk3n TAAGGATTTGGTTTTGCTTCCTTGAAGAACAG(+CAGTGT)GATCCAGAGGTCAGGGCGAGTTCAAGTTCTA NGG 10 8 17 8 24 0 100 5 10
    240605225142_hutj GCCAGCCCAGAGCCGGGCCACTCCCCTTTCTG(+CAGTGT)GTACCAAGAAAAGCTTTCCTTGATGACCAGT NGG 10 8 17 8 24 0 100 5 10
    240605225141_eiaw AGGGCACTGGTCATCAAGGAAAGCTTTTCTTG(+CAGTGT)GTACCAGAAAGGGGAGTGGCCCGGCTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605225139_0571 TCAGTGCCGCTCAGCCTTGGCCCTGTGTGGCG(+CAGTGT)GATCCTGACCTAGAGAACAGGTGTGGGGACA NGG 10 8 17 8 24 0 100 5 10
    240605225137_1tnp TGCAGTGTCCCCACACCTGTTCTCTAGGTCAG(+CAGTGT)GATCCGCCACACAGGGCCAAGGCTGAGCGGC NGG 10 8 17 8 24 0 100 5 10
    240605225136_znkz GCCTTTGCCTACAGAGACATGAGGCTGAGCTG(+CAGTGT)GATCCTGACAATACTGTCCATTTGCCTGAGT NGG 10 8 17 8 24 0 100 5 10