Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225023_z66l ATTATGCTTTGATGTAGAGCCAGCTTGGAGGG(+CAGTGT)GATCCCCAAAGCTGCTCACTGTTTTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605225021_fsjd CCAGCTCTAGAAAAACAGTGAGCAGCTTTGGG(+CAGTGT)GATCCCCTCCAAGCTGGCTCTACATCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605225019_h174 ATTATGCTTTGATGTAGAGCCAGCTTGGAGGG(+CAGTGT)GATCCCCAAAGCTGCTCACTGTTTTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605225017_aui2 GCCTTCTTGGGACAAGGAAGAGAAGAAGGAAG(+CAGTGT)GATCCAAGCAAAAGGTGGTCTCTCCTGCTCG NGG 10 8 17 8 24 0 100 5 10
    240605225016_nnvy GGGGCCGAGCAGGAGAGACCACCTTTTGCTTG(+CAGTGT)GATCCTTCCTTCTTCTCTTCCTTGTCCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605225015_immy ACTCTGTAGAACAGTCTGGCCTTGAACTCAAG(+CAGTGT)GATCCACCTGCTTCTGCCTCTTGGGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605225013_2012 TAATACCAGCACCCAAGAGGCAGAAGCAGGTG(+CAGTGT)GATCCTTGAGTTCAAGGCCAGACTGTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240605225011_tvrv TGGTGTGATGCCTTCTTGTAGTGTTCTCTGGG(+CAGTGT)GATCCCTCATACCCCAGCCACAGCACAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605225009_jk3d TCTGGAAACTGTGCTGTGGCTGGGGTATGAGG(+CAGTGT)GATCCCCAGAGAACACTACAAGAAGGCATCA NGG 10 8 17 8 24 0 100 5 10
    240605225008_n9pb TACCCCTCCTGTGTGTGAAGGCTCTGCTGAAG(+CAGTGT)GTACCTTTGGGTCTGGTCTGACCTTACTGCT NGG 10 8 17 8 24 0 100 5 10