Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605224915_ttcp ACACCCATTCTTCCATGACAGTCTGGACAGAG(+CAGTGT)GATCCAAGATGGCTGATGAGAAATCCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605224914_qui1 TCCCCAGTGGCCCAGGTCCAGATCCTTGTGTG(+CAGTGT)GATCCAAAGCAAATCAGCACAGATTCCACAG NGG 10 8 17 8 24 0 100 5 10
    240605224912_fv3t GGTGTCTGTGGAATCTGTGCTGATTTGCTTTG(+CAGTGT)GATCCACACAAGGATCTGGACCTGGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240605224911_wgme GTGCTAGCTGCTGCCACCCCTGAGCCGTCGTG(+CAGTGT)GATCCCACTGTCGCGCACAGCACCTGTGGAC NGG 10 8 17 8 24 0 100 5 10
    240605224909_2csb AGGGAGTCCACAGGTGCTGTGCGCGACAGTGG(+CAGTGT)GATCCACGACGGCTCAGGGGTGGCAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605224907_nkmm CTAGGAGGTGCCCAGAGAAGGACAACGTGGGG(+CAGTGT)GTACCTCAGAGTGGGTAGAGATGCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240605224905_wd88 CCACAGCCTGTGGCATCTCTACCCACTCTGAG(+CAGTGT)GTACCCCCACGTTGTCCTTCTCTGGGCACCT NGG 10 8 17 8 24 0 100 5 10
    240605224903_1cp0 ATTGTGGGAATAAAGCCTCTGGCTGAGATATG(+CAGTGT)GTACCCCATGGGCTTCTTGCAAGGGAATCCT NGG 10 8 17 8 24 0 100 5 10
    240605224901_2ksy ATTTGAGGATTCCCTTGCAAGAAGCCCATGGG(+CAGTGT)GTACCATATCTCAGCCAGAGGCTTTATTCCC NGG 10 8 17 8 24 0 100 5 10
    240605224858_qa31 TTGCCTTCCAGAAAGCCTCCATCTAGCCCCAG(+CAGTGT)GTACCCCCAGGACTCCAGCCTTTAAAGAACT NGG 10 8 17 8 24 0 100 5 10