Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222630_422c CCCCACAGAGAACATGAAGTCACAGTGGAGTG(+CAGTGT)GATCCCCTGTTTCTTAGGTATTTGATCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605222628_p0km GAACTCTCTGATCAAATACCTAAGAAACAGGG(+CAGTGT)GATCCACTCCACTGTGACTTCATGTTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605222627_njtg GAAACTGAGGGAATTTGTGGCCTTAAAGCAGG(+CAGTGT)GATCCCAGAGTAAAGCTCTCAGGGTTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605222625_h8tj AAGGACCCAGAACCCTGAGAGCTTTACTCTGG(+CAGTGT)GATCCCTGCTTTAAGGCCACAAATTCCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605222623_q018 GAGGCTGGCGGAGGCTGGTGTCTCATCATCGG(+CAGTGT)GATCCCCTCCCCAGGACCAGGAGGGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605222621_w2yq GTCTTCTGCAGCCCTCCTGGTCCTGGGGAGGG(+CAGTGT)GATCCCGATGATGAGACACCAGCCTCCGCCA NGG 10 8 17 8 24 0 100 5 10
    240605222619_x8nf CTAGTCTGAAGCCCAGCCCTCCACATCTGGGG(+CAGTGT)GTACCAAACCTGCCTTGGGAACTGCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605222618_esr5 CTTACTCAGGAGCAGTTCCCAAGGCAGGTTTG(+CAGTGT)GTACCCCCAGATGTGGAGGGCTGGGCTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605222616_9z0n TTGAGTACACACCACAGGGGGAGTGCTACAGG(+CAGTGT)GTACCAGGCTCTGGTGGAAACAACTGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605222614_zew7 AACAGGGCACCAGTTGTTTCCACCAGAGCCTG(+CAGTGT)GTACCCTGTAGCACTCCCCCTGTGGTGTGTA NGG 10 8 17 8 24 0 100 5 10