Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222521_ednb AGGATTTGAACTTGGGCCCTCTGTAAGAAGAG(+CAGTGT)GATCCATTCTTAACTCCTGGACCATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240605222520_etjx AGGCTAATGAGATGGTCCAGGAGTTAAGAATG(+CAGTGT)GATCCTCTTCTTACAGAGGGCCCAAGTTCAA NGG 10 8 17 8 24 0 100 5 10
    240605222518_82r8 ACGAGTGCTGAGGATCTCAGCAGGTGGATGGG(+CAGTGT)GATCCCAGGGATGCAAGAGCTTTCACCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605222515_vuvy GGTGCAGAGGGTGAAAGCTCTTGCATCCCTGG(+CAGTGT)GATCCCCATCCACCTGCTGAGATCCTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605222513_eo44 AAAACAAAAAACCCCAAAACTTCCTACTGCAG(+CAGTGT)GTACCCCTTCATTACAAAAGTATGCAAACCA NGG 10 8 17 8 24 0 100 5 10
    240605222511_aoic TGAGATGGTTTGCATACTTTTGTAATGAAGGG(+CAGTGT)GTACCTGCAGTAGGAAGTTTTGGGGTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605222510_xmrq CACATGCTCCACCCTGTCTTGCCCTGCTGAAG(+CAGTGT)GTACCACTCAGGCATCAGGGCTGGGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605222509_frnv GAGAGCAAGCACCCAGCCCTGATGCCTGAGTG(+CAGTGT)GTACCTTCAGCAGGGCAAGACAGGGTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240605222507_qp3n CCAACCCCAAAGAAGCATTGCACAGACAGAGG(+CAGTGT)GTACCCTTTCCCAGCTGAAGGTCACCAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605222505_12q8 CCTGCCTCTTGGTGACCTTCAGCTGGGAAAGG(+CAGTGT)GTACCCTCTGTCTGTGCAATGCTTCTTTGGG NGG 10 8 17 8 24 0 100 5 10