Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222504_3188 TGCAGAAAGTATTGTGGGCATCTATTTAGATG(+CAGTGT)GATCCAAGGCAATAGAATGGTCAAGAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605222502_w3ac ATGTGGGCTTTCTTGACCATTCTATTGCCTTG(+CAGTGT)GATCCATCTAAATAGATGCCCACAATACTTT NGG 10 8 17 8 24 0 100 5 10
    240605222500_d36e ATTCCGTAGTGTTAAATACCCTCCAGGAGATG(+CAGTGT)GATCCCTACATAAAACATTCACACATTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605222458_wfe0 GTGAGAGAGAATGTGTGAATGTTTTATGTAGG(+CAGTGT)GATCCATCTCCTGGAGGGTATTTAACACTAC NGG 10 8 17 8 24 0 100 5 10
    240605222457_alpk AAAAGCCTGTTTTCACAATACCAGCAGAGCAG(+CAGTGT)GTACCCACATACATCCTCCTGCAGACGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605222456_9t2m GTGAACTCCCGTCTGCAGGAGGATGTATGTGG(+CAGTGT)GTACCTGCTCTGCTGGTATTGTGAAAACAGG NGG 10 8 17 8 24 0 100 5 10
    240605222454_bsfc TTTCTCCATACACACATTTATATTTAGAGAGG(+CAGTGT)GATCCAAACCAGACATGCTGATGTTGGATCT NGG 10 8 17 8 24 0 100 5 10
    240605222453_tdos ACCTTAGATCCAACATCAGCATGTCTGGTTTG(+CAGTGT)GATCCCTCTCTAAATATAAATGTGTGTATGG NGG 10 8 17 8 24 0 100 5 10
    240605222451_l9xp GTGACATGTACAATATATAGAGGCCCCCACAG(+CAGTGT)GATCCCCTGCTCCTGGACCTGAGCCCAACCA NGG 10 8 17 8 24 0 100 5 10
    240605222449_xvmk GCACCTGGTTGGGCTCAGGTCCAGGAGCAGGG(+CAGTGT)GATCCTGTGGGGGCCTCTATATATTGTACAT NGG 10 8 17 8 24 0 100 5 10