Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222427_hspg GGTGAGAAGGGTGTGGCTCGGATGGCATCCTG(+CAGTGT)GTACCCACAGTGAGCTGGTAGAATACTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605222426_z7gs AGATTACTTAGTATTCTACCAGCTCACTGTGG(+CAGTGT)GTACCAGGATGCCATCCGAGCCACACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605222424_m0q2 GAAAGGCCCAGTGTGGTACCAGACACACAGTG(+CAGTGT)GATCCTTCCAGAGGTGCAGGTCTGTTTATTA NGG 10 8 17 8 24 0 100 5 10
    240605222422_jlt0 ACCACTAATAAACAGACCTGCACCTCTGGAAG(+CAGTGT)GATCCACTGTGTGTCTGGTACCACACTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605222421_59w2 AGCTCTACTGGGGCCCTCTTGACCAGGAGTGG(+CAGTGT)GTACCAGGGGTCATTGCCAAAGTTTGAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605222418_unnt CAAGAGTCCTCAAACTTTGGCAATGACCCCTG(+CAGTGT)GTACCCACTCCTGGTCAAGAGGGCCCCAGTA NGG 10 8 17 8 24 0 100 5 10
    240605222415_nbu9 TGGTCCTTCTCAGGCACTGTGGGGCCCTGGGG(+CAGTGT)GATCCTCGTTCCTGGCCTTGCCCTTCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605222414_404g CCCTAGCCAGGAAGGGCAAGGCCAGGAACGAG(+CAGTGT)GATCCCCCAGGGCCCCACAGTGCCTGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605222412_zges AGAGGGCACCGGTGGGAGTCCTGGGGTTGGTG(+CAGTGT)GTACCTTCATGTGCTCAGCATCTCGCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605222411_aj6q GTCAAGGCTGGCGAGATGCTGAGCACATGAAG(+CAGTGT)GTACCACCAACCCCAGGACTCCCACCGGTGC NGG 10 8 17 8 24 0 100 5 10