Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222333_lhhu TGAAGGGTCTGACAACCAGACCTTACCAGGTG(+CAGTGT)GTACCGGTTTCATGCCAGAGTCAGGTTAAGA NGG 10 8 17 8 24 0 100 5 10
    240605222331_y0nk TACGCTCTTAACCTGACTCTGGCATGAAACCG(+CAGTGT)GTACCACCTGGTAAGGTCTGGTTGTCAGACC NGG 10 8 17 8 24 0 100 5 10
    240605222329_v3pe TCTACTTGCTTTGCATGCAGCCCTGAGGAAGG(+CAGTGT)GATCCTGATTTCCAACCCTTTGCTCTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605222327_pgfw TCCCTCAGAGAGAGCAAAGGGTTGGAAATCAG(+CAGTGT)GATCCCTTCCTCAGGGCTGCATGCAAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605222326_sf4w ACGGAGTTTTGTTTTCTCTGCCAGAAGGCAAG(+CAGTGT)GATCCAAGAGTTCCCGGTATCAGCCTACTCC NGG 10 8 17 8 24 0 100 5 10
    240605222325_2c87 GGAGGGGAGTAGGCTGATACCGGGAACTCTTG(+CAGTGT)GATCCTTGCCTTCTGGCAGAGAAAACAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605222323_i3o6 AGTAAGACCTTCCCTAGGGAACAGACAGCTGG(+CAGTGT)GTACCTGGGTGCCAGGCAAGACAAGACTCTT NGG 10 8 17 8 24 0 100 5 10
    240605222321_yw5s CTTGTAAGAGTCTTGTCTTGCCTGGCACCCAG(+CAGTGT)GTACCCAGCTGTCTGTTCCCTAGGGAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605222319_0zg6 CTGTCCAGAGGCTCCCTCCCCTCCTCTTACAG(+CAGTGT)GATCCCTAGAGCAGCTCAGCCCTGTCGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605222317_5ibe TCACGACACCGACAGGGCTGAGCTGCTCTAGG(+CAGTGT)GATCCTGTAAGAGGAGGGGAGGGAGCCTCTG NGG 10 8 17 8 24 0 100 5 10