Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222307_hev8 AGATGCTCCGAAAGACCTTCCCGAGCTCCAGG(+CAGTGT)GTACCCTGGCCCAACCAAACTACTCTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605222304_7e4r TTGAGAGCAGGCCCTGATCATTCTGCCCCAGG(+CAGTGT)GATCCTAAGGCCTCACGTAGCACTGCCGGCA NGG 10 8 17 8 24 0 100 5 10
    240605222302_c35f ATCGCTGCCGGCAGTGCTACGTGAGGCCTTAG(+CAGTGT)GATCCCTGGGGCAGAATGATCAGGGCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605222300_4rym AGGAGATATCTCAACTGCTGTACCCAGTAAGG(+CAGTGT)GATCCTTCTCTACAGACCCTCACTTGGCTAG NGG 10 8 17 8 24 0 100 5 10
    240605222258_1o0g TTTCTCTAGCCAAGTGAGGGTCTGTAGAGAAG(+CAGTGT)GATCCCTTACTGGGTACAGCAGTTGAGATAT NGG 10 8 17 8 24 0 100 5 10
    240605222256_pzuv GGCTCAGTAATTAGAGCACTGGGCCTTCCAGG(+CAGTGT)GTACCCAAGTTCAGTTTTCAGTATCTATATG NGG 10 8 17 8 24 0 100 5 10
    240605222254_t2yz GCCATCATATAGATACTGAAAACTGAACTTGG(+CAGTGT)GTACCCTGGAAGGCCCAGTGCTCTAATTACT NGG 10 8 17 8 24 0 100 5 10
    240605222253_zq14 CTTTTGATCATTTCTCTAGAGGTCTGGGTGTG(+CAGTGT)GATCCTTTTTTGCCTCAGATGTGGGTGGCGA NGG 10 8 17 8 24 0 100 5 10
    240605222251_4arf GTCTCTCGCCACCCACATCTGAGGCAAAAAAG(+CAGTGT)GATCCACACCCAGACCTCTAGAGAAATGATC NGG 10 8 17 8 24 0 100 5 10
    240605222250_htmf AACAAGGAAGTTCTGGAACCGCCCACATAGAG(+CAGTGT)GATCCGCCCCTCAATCGAGGACTCTGTGTGT NGG 10 8 17 8 24 0 100 5 10