Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222159_7jt0 TAGTTCACTGCCAACCATTGGACTGAGCACGG(+CAGTGT)GATCCCGAATGGGGTCCCCAATGGAGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240605222158_d8rl TTTCTAACTCCTCCATTGGGGACCCCATTCGG(+CAGTGT)GATCCCGTGCTCAGTCCAATGGTTGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605222156_cjwq CACAGTCCATATAGTTGTTTCTTTCCAGTCTG(+CAGTGT)GATCCTATAATGTATTTTAAGATGTGACAGT NGG 10 8 17 8 24 0 100 5 10
    240605222155_ydfp ATTGAACTGTCACATCTTAAAATACATTATAG(+CAGTGT)GATCCAGACTGGAAAGAAACAACTATATGGA NGG 10 8 17 8 24 0 100 5 10
    240605222154_hkvj ACTGAGTGTCCTCACCAACTGTACCATCAGTG(+CAGTGT)GTACCAAAACTGACTGGTTTTAAAAAACACG NGG 10 8 17 8 24 0 100 5 10
    240605222152_1kdg ATCCACGTGTTTTTTAAAACCAGTCAGTTTTG(+CAGTGT)GTACCACTGATGGTACAGTTGGTGAGGACAC NGG 10 8 17 8 24 0 100 5 10
    240605222151_3quv ATAAGGGCCCTGGACAGGCACAGATACTCTTG(+CAGTGT)GATCCCCAGTGGAGATGGATAAGAACCTCAA NGG 10 8 17 8 24 0 100 5 10
    240605222149_7kuk CCTCTTTGAGGTTCTTATCCATCTCCACTGGG(+CAGTGT)GATCCAAGAGTATCTGTGCCTGTCCAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240605222146_rj5g GGATGGACCATCCAGAGACTACCCCACCCGGG(+CAGTGT)GATCCATCCCATAATCAGCCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605222145_ed6v AGTGTCTGGGTTTGGTGGCTGATTATGGGATG(+CAGTGT)GATCCCCGGGTGGGGTAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10