Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222049_hilu ATGCCAAGCTTAACATACGCGGACCCTCACAG(+CAGTGT)GATCCCTAGATCAACCACCCTGAACTGAACT NGG 10 8 17 8 24 0 100 5 10
    240605222047_nn38 GTTCCAGTTCAGTTCAGGGTGGTTGATCTAGG(+CAGTGT)GATCCTGTGAGGGTCCGCGTATGTTAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605222046_twjk GAAGATCTCCCTAGAGGCCACTCCTTTAGGAG(+CAGTGT)GTACCAGGAACGCTTTCTGGTGTTGTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240605222044_cgmu AGCAGCCCTAACAACACCAGAAAGCGTTCCTG(+CAGTGT)GTACCTCCTAAAGGAGTGGCCTCTAGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605222042_i1tj GGGTCACTCATGAATAAGCACACCTGGGGAAG(+CAGTGT)GTACCAGGCAATAGTCCTGTCCCTTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240605222040_3lyh GGTAGGCCAGCAAGGGACAGGACTATTGCCTG(+CAGTGT)GTACCTTCCCCAGGTGTGCTTATTCATGAGT NGG 10 8 17 8 24 0 100 5 10
    240605222039_7c1k TCACACATTCCAGAGACATCAACCCTGCAGTG(+CAGTGT)GATCCAGTGTGATTTCCAGACTCTCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605222038_q6ay TTTTGTGCCTGGAGAGTCTGGAAATCACACTG(+CAGTGT)GATCCACTGCAGGGTTGATGTCTCTGGAATG NGG 10 8 17 8 24 0 100 5 10
    240605222035_z2yj ACATCAGGCACCAGTGAAGATACATCCCCCTG(+CAGTGT)GATCCCATTGATGAGGACTCTAAAAGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240605222033_w7d1 CTTCTATCCCCTTTTAGAGTCCTCATCAATGG(+CAGTGT)GATCCAGGGGGATGTATCTTCACTGGTGCCT NGG 10 8 17 8 24 0 100 5 10