Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222009_x6p5 AGCCACAGCTGGCTTTCCACACAGGTTCTAGG(+CAGTGT)GATCCAAACAGGGCCTCAGGTGTATTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605222008_nuq4 AACCCTGACTAATACACCTGAGGCCCTGTTTG(+CAGTGT)GATCCCTAGAACCTGTGTGGAAAGCCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605222007_mkoe CATGTTTATAAAATAGCACGTACTGAAAACTG(+CAGTGT)GTACCACCCGTGACTGTATAAAGAAGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605222005_bv2v AGTAATCTACCTTCTTTATACAGTCACGGGTG(+CAGTGT)GTACCAGTTTTCAGTACGTGCTATTTTATAA NGG 10 8 17 8 24 0 100 5 10
    240605222003_16q3 GAGGCCAAAATTAGTTTTTGCTACTTGATTTG(+CAGTGT)GTACCTTAGGGTTTGGGCTCCTCTGGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605222001_91cl CAAGAAAAAACCAGAGGAGCCCAAACCCTAAG(+CAGTGT)GTACCAAATCAAGTAGCAAAAACTAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240605221959_2yep GATAGCAAAACAAAGGCACCTCTACACTCCAG(+CAGTGT)GTACCCCACATACATAAACCATTGTTGATCC NGG 10 8 17 8 24 0 100 5 10
    240605221957_w18a CCTACGGATCAACAATGGTTTATGTATGTGGG(+CAGTGT)GTACCTGGAGTGTAGAGGTGCCTTTGTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605221955_l7r9 ATTGTCATGGGGCTCAGGGATACAGATAACAG(+CAGTGT)GATCCTAGAAGCAGGCGTGATATTCCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605221954_21rp CCTGGTCCTTGGAATATCACGCCTGCTTCTAG(+CAGTGT)GATCCTGTTATCTGTATCCCTGAGCCCCATG NGG 10 8 17 8 24 0 100 5 10