Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222029_181v TCAAATCGATTTTGTTCTTGATTTTTATGAAG(+CAGTGT)GATCCATCTCTGTGTATTGAGGGGTAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605222026_g8lh AAATCATTTTTACCCCTCAATACACAGAGATG(+CAGTGT)GATCCTTCATAAAAATCAAGAACAAAATCGA NGG 10 8 17 8 24 0 100 5 10
    240605222025_x8ci GCTAGAACTTACAGAGGCTGTGGTTGATCTGG(+CAGTGT)GTACCATGTGCCATGATGTTTTACTGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605222023_poso TAAAAGCTGGCAGTAAAACATCATGGCACATG(+CAGTGT)GTACCCAGATCAACCACAGCCTCTGTAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605222021_mova AGATTAAAACCCTCTGCAAGCGCACAGCCTTG(+CAGTGT)GTACCGCCTCTCATCTCAGTTAACAGTTAGA NGG 10 8 17 8 24 0 100 5 10
    240605222019_q2br TGTCATCTAACTGTTAACTGAGATGAGAGGCG(+CAGTGT)GTACCAAGGCTGTGCGCTTGCAGAGGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605222017_tnvd ACAGTGTGCTCCTGTCTCTGTCACAGTTCCCG(+CAGTGT)GATCCCTCAGCAGCCCTGTGTGTTGATAAAG NGG 10 8 17 8 24 0 100 5 10
    240605222015_ykep GCAAACTTTATCAACACACAGGGCTGCTGAGG(+CAGTGT)GATCCGGGAACTGTGACAGAGACAGGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605222014_mis9 AGTACAAGGGCCCTCAGAAGGTGGAGGCATTG(+CAGTGT)GATCCCCTGGAGCTGGAGTTATGGATATGGC NGG 10 8 17 8 24 0 100 5 10
    240605222011_hy41 CGGCAGCCATATCCATAACTCCAGCTCCAGGG(+CAGTGT)GATCCAATGCCTCCACCTTCTGAGGGCCCTT NGG 10 8 17 8 24 0 100 5 10