Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222108_iqld CAGGCTACCATACACACTCAGCATTTGTGTGG(+CAGTGT)GTACCGGGGAACCCTGCTTCTCACTCTTGCA NGG 10 8 17 8 24 0 100 5 10
    240605222104_anql TGCCATGCAAGAGTGAGAAGCAGGGTTCCCCG(+CAGTGT)GTACCCACACAAATGCTGAGTGTGTATGGTA NGG 10 8 17 8 24 0 100 5 10
    240605222103_5v7g AATCTTGGACCTGTTTCATGAAGACCCAGTAG(+CAGTGT)GTACCCGGATTCTGGCTAGGTTCTCATTCAG NGG 10 8 17 8 24 0 100 5 10
    240605222101_cmu2 ACTTGCTGAATGAGAACCTAGCCAGAATCCGG(+CAGTGT)GTACCTACTGGGTCTTCATGAAACAGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605222058_y7yf ACAGTCTGGGTCTTTATTGATTGACAGTCTGG(+CAGTGT)GTACCACCACACACACAGACTTCAGGTATTT NGG 10 8 17 8 24 0 100 5 10
    240605222057_e5nc TTAAGAAATACCTGAAGTCTGTGTGTGTGGTG(+CAGTGT)GTACCCAGACTGTCAATCAATAAAGACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605222056_v3yu GGGAGTCGTCTGCTTCACATGGATATGCGACG(+CAGTGT)GTACCTTCTGACAGGGGGTTTTCACCCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605222054_hcic GTTTTCAAGGGGTGAAAACCCCCTGTCAGAAG(+CAGTGT)GTACCGTCGCATATCCATGTGAAGCAGACGA NGG 10 8 17 8 24 0 100 5 10
    240605222053_pn7y GAAGATCTCCCTAGAGGCCACTCCTTTAGGAG(+CAGTGT)GTACCAGGAACGCTTTCTGGTGTTGTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240605222051_ased AGCAGCCCTAACAACACCAGAAAGCGTTCCTG(+CAGTGT)GTACCTCCTAAAGGAGTGGCCTCTAGGGAGA NGG 10 8 17 8 24 0 100 5 10