Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222722_57f9 CCCCGTGACTGTGTGGAGCAAAGCCGGGGCAG(+CAGTGT)GTACCCGAGCTTTGCGATCCCAACAGGAGCG NGG 10 8 17 8 24 0 100 5 10
    240605222720_rews CAGCTCGCTCCTGTTGGGATCGCAAAGCTCGG(+CAGTGT)GTACCTGCCCCGGCTTTGCTCCACACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605222718_qrzq AGACAGCTGTTCCCTATCTGCAGGTGGCCTAG(+CAGTGT)GTACCACTGTGAAGACAGATGCCTGTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605222716_wpu9 TTCTTAGGAAACAGGCATCTGTCTTCACAGTG(+CAGTGT)GTACCTAGGCCACCTGCAGATAGGGAACAGC NGG 10 8 17 8 24 0 100 5 10
    240605222715_9g9a TCTTGGGTTGGGGAGATGAGACCCGAGTTTAG(+CAGTGT)GTACCACCCAAACATACAGAACAAAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605222713_tv6w TATGGCCTTCCTTTGTTCTGTATGTTTGGGTG(+CAGTGT)GTACCTAAACTCGGGTCTCATCTCCCCAACC NGG 10 8 17 8 24 0 100 5 10
    240605222711_5rxh GGGTATACAATGCCCTCTCCTGTTCTCTGTGG(+CAGTGT)GTACCTGCAGTCATGTGCACATACCTGCACG NGG 10 8 17 8 24 0 100 5 10
    240605222710_9ohb ACACACGTGCAGGTATGTGCACATGACTGCAG(+CAGTGT)GTACCCACAGAGAACAGGAGAGGGCATTGTA NGG 10 8 17 8 24 0 100 5 10
    240605222708_99i4 CGTGGTAAGGCGAGCCTTCCTTTGTGGGACAG(+CAGTGT)GATCCTTCACTTCATCTTTTCCAAAAACTGT NGG 10 8 17 8 24 0 100 5 10
    240605222706_t017 CACACACAGTTTTTGGAAAAGATGAAGTGAAG(+CAGTGT)GATCCTGTCCCACAAAGGAAGGCTCGCCTTA NGG 10 8 17 8 24 0 100 5 10