Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222824_9kc8 GGTACTGCTCAGGCTAATAAGAAGTGAAGTCG(+CAGTGT)GATCCAGAGCCTGATTCCCTTGCAAGCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605222823_lbe3 TGCGCACTGGCTTGCAAGGGAATCAGGCTCTG(+CAGTGT)GATCCGACTTCACTTCTTATTAGCCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240605222821_ausy AAGCCCATTCCACAGAAGTGAGTTCACATTTG(+CAGTGT)GTACCGTGAGCCTAGTCAAAAGCTTGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605222820_1q67 TCCCTAGCTGCAAGCTTTTGACTAGGCTCACG(+CAGTGT)GTACCAAATGTGAACTCACTTCTGTGGAATG NGG 10 8 17 8 24 0 100 5 10
    240605222819_5ylv AGCATCTTCACTCTTCTGGCCTACCAAGCTTG(+CAGTGT)GTACCTATTTCTCATCATAACTTTGGAATGT NGG 10 8 17 8 24 0 100 5 10
    240605222817_3zhj ACCCTACATTCCAAAGTTATGATGAGAAATAG(+CAGTGT)GTACCAAGCTTGGTAGGCCAGAAGAGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605222815_mved ACATGCCTTTGGGTGTCTGTTTGCCAGGCTGG(+CAGTGT)GATCCCGGGCTCCTGAGTGAGCAGTAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605222812_zwbh ACAGTCCAGCTACTGCTCACTCAGGAGCCCGG(+CAGTGT)GATCCCAGCCTGGCAAACAGACACCCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605222811_m0pc AAGATAACCAAGGCTTCCACTGGCAGGGTTTG(+CAGTGT)GATCCCAGGCAGGCAAGCAGGCAGGCTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605222809_9qem CCATCCACCAGCCTGCCTGCTTGCCTGCCTGG(+CAGTGT)GATCCAAACCCTGCCAGTGGAAGCCTTGGTT NGG 10 8 17 8 24 0 100 5 10