Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222842_b2qt GTGGATGGCACTGGAGCTGTGAGTGAAATGGG(+CAGTGT)GTACCAGGGCAGGAGACTGCGCCCACAATGT NGG 10 8 17 8 24 0 100 5 10
    240605222840_gr0w GGAGAACATTGTGGGCGCAGTCTCCTGCCCTG(+CAGTGT)GTACCCCATTTCACTCACAGCTCCAGTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605222837_r51w ATGGGTCATAAGTGGGAGAGCTGGAGGCCCAG(+CAGTGT)GTACCACCCTCCCCCTGGGCTATCCCGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605222836_i1s0 AGGGGGTGCCGGGATAGCCCAGGGGGAGGGTG(+CAGTGT)GTACCTGGGCCTCCAGCTCTCCCACTTATGA NGG 10 8 17 8 24 0 100 5 10
    240605222835_3ya9 AGCGGCTAAGAGCACTGGCCTCTTGTGCAGAG(+CAGTGT)GATCCGTTTGATTCCTAGCATCCACAAGGTA NGG 10 8 17 8 24 0 100 5 10
    240605222833_eg18 GAAGCTACCTTGTGGATGCTAGGAATCAAACG(+CAGTGT)GATCCTCTGCACAAGAGGCCAGTGCTCTTAG NGG 10 8 17 8 24 0 100 5 10
    240605222832_zn7w TGTCCACCCACTTTCATGACAACCCAATTTGG(+CAGTGT)GATCCCCATGGCAAGGGTGGGGTAGTACAGA NGG 10 8 17 8 24 0 100 5 10
    240605222830_ebfl TTATTTCTGTACTACCCCACCCTTGCCATGGG(+CAGTGT)GATCCCAAATTGGGTTGTCATGAAAGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605222827_lgxb GACTAACACCTTCTATAACCCCAGCCCCTAGG(+CAGTGT)GATCCGACAACCTCTAGCTTCCGCGGGCACC NGG 10 8 17 8 24 0 100 5 10
    240605222826_kcx8 GTTCAGGTGCCCGCGGAAGCTAGAGGTTGTCG(+CAGTGT)GATCCCTAGGGGCTGGGGTTATAGAAGGTGT NGG 10 8 17 8 24 0 100 5 10