Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222751_shnu TTACTTCCTCTTGATTCATTTTTTGAGTGTAG(+CAGTGT)GTACCTTATAGTTCTGTCAAAGGTAGACAGA NGG 10 8 17 8 24 0 100 5 10
    240605222750_kdmz TCAACTCTGTCTACCTTTGACAGAACTATAAG(+CAGTGT)GTACCTACACTCAAAAAATGAATCAAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605222748_gan2 AAATCTTTTTAAAAAAGCAAACAAAAAAATGG(+CAGTGT)GTACCAGATGAAAGGGGTTCAGGACATGGTT NGG 10 8 17 8 24 0 100 5 10
    240605222747_qpuu GAAGCAACCATGTCCTGAACCCCTTTCATCTG(+CAGTGT)GTACCCATTTTTTTGTTTGCTTTTTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605222746_71lw GTAAAATTTTATACAACCAAGTGTTCTTTCTG(+CAGTGT)GATCCTGGACATTATGGTTAGGCACCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240605222744_hqs3 CCATGACCCTGGTGCCTAACCATAATGTCCAG(+CAGTGT)GATCCAGAAAGAACACTTGGTTGTATAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605222742_6x7q GCTTAAGATTTTGCTACAGAGACAAAAAGGTG(+CAGTGT)GATCCTTTCCCTGCCCAGCTACTCTGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240605222741_49f9 CCCTGGTGGACAGAGTAGCTGGGCAGGGAAAG(+CAGTGT)GATCCACCTTTTTGTCTCTGTAGCAAAATCT NGG 10 8 17 8 24 0 100 5 10
    240605222740_7cgf AGTGGTTAAGAGCACTGACTGTTCTTAGAGAG(+CAGTGT)GATCCAGGTTTGATTCCCAACACCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240605222738_bxfu GCTTTCCATGTGGGTGTTGGGAATCAAACCTG(+CAGTGT)GATCCTCTCTAAGAACAGTCAGTGCTCTTAA NGG 10 8 17 8 24 0 100 5 10