Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222807_f2ri CTGCCTCAGCCTGTGCTCCAGGTTGGTTCCAG(+CAGTGT)GTACCCTAGCGGCCTTGGCAGGTTTTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605222805_apkc GATGTGCTGCAAAACCTGCCAAGGCCGCTAGG(+CAGTGT)GTACCTGGAACCAACCTGGAGCACAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605222804_gk54 TAATGAGCTTCCTTTTCTCCAATGTGTATGAG(+CAGTGT)GTACCTGTATGATGTGCTCTGATCACACCCA NGG 10 8 17 8 24 0 100 5 10
    240605222803_f7id AAGGGTGGGTGTGATCAGAGCACATCATACAG(+CAGTGT)GTACCTCATACACATTGGAGAAAAGGAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605222801_1ex9 GTTTCTAACAGAAATTACGATTTGAGGGTCAG(+CAGTGT)GATCCTGGGAGAGACTCTCCAGCTCCAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605222758_6zc1 AATCTGTCCTGGAGCTGGAGAGTCTCTCCCAG(+CAGTGT)GATCCTGACCCTCAAATCGTAATTTCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605222757_pnac TGAACTTCACCCTGAAAGAGTGAGCTGTGCTG(+CAGTGT)GATCCTCCCCGTAAGAAATTCTATAGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605222755_pn0e AATCACTTCTCTATAGAATTTCTTACGGGGAG(+CAGTGT)GATCCAGCACAGCTCACTCTTTCAGGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605222754_s2e9 AAGGATTCCGAAGAATAAGTTACAGCTGTCTG(+CAGTGT)GATCCTGCTTGGGAATTTTTAAGGGATATGG NGG 10 8 17 8 24 0 100 5 10
    240605222752_c516 CTTTCCCATATCCCTTAAAAATTCCCAAGCAG(+CAGTGT)GATCCAGACAGCTGTAACTTATTCTTCGGAA NGG 10 8 17 8 24 0 100 5 10