Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222916_eznq TGCACCTCTTCAAAAAAGAAATCTTTCTTTGG(+CAGTGT)GATCCCACATTCCCTCAGCCTTATCAACCCT NGG 10 8 17 8 24 0 100 5 10
    240605222915_tadp GAGACAGGGTTGATAAGGCTGAGGGAATGTGG(+CAGTGT)GATCCCAAAGAAAGATTTCTTTTTTGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605222913_kcsz AAGGGCACAGAAAACTCGAAGCCCTTCCAGTG(+CAGTGT)GTACCAGCTCACTCCCTGAGGAGAGGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605222911_2ed8 CCTTGACAACCCTCTCCTCAGGGAGTGAGCTG(+CAGTGT)GTACCACTGGAAGGGCTTCGAGTTTTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605222910_6y9d AATGAAGAGGATATGGGGGAAACCCTCACGAG(+CAGTGT)GTACCCTCGGGGAAAGAGGACTGGGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605222908_txhc ATTCCTGCACTCCCAGTCCTCTTTCCCCGAGG(+CAGTGT)GTACCTCGTGAGGGTTTCCCCCATATCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605222906_l0xz CTGTGGCCTGCAAGGCCCATCTCAGGTTTGGG(+CAGTGT)GATCCCCCAGTCCCTATGATCAGTGTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605222903_z8f0 GGATACCCCAACACTGATCATAGGGACTGGGG(+CAGTGT)GATCCCCAAACCTGAGATGGGCCTTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605222901_ngkw GGGAGGAAATGAAAATTGGCTCAGAGACGGAG(+CAGTGT)GTACCTCAAAAAAAAAAAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605222900_n8d8 ATTTCTTTTTTTTTTTTTTTTTTTTTTTTGAG(+CAGTGT)GTACCTCCGTCTCTGAGCCAATTTTCATTTC NGG 10 8 17 8 24 0 100 5 10