Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223032_fhtl TTCCCTGAGACTTGGTACTCTATGAGGTGGAG(+CAGTGT)GTACCCATCCCTGATCTGAACCCTTTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605223030_2qbc CTAACCCAGAAAAGGGTTCAGATCAGGGATGG(+CAGTGT)GTACCTCCACCTCATAGAGTACCAAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605223027_y195 CAGACGCAGACGGGGCAGCAAGGACGGCCCTG(+CAGTGT)GATCCAACTCTTCAGCTACTGCTCCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605223026_q0l1 GGCTTGGCTTGGGAGCAGTAGCTGAAGAGTTG(+CAGTGT)GATCCAGGGCCGTCCTTGCTGCCCCGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605223024_yk9a TCCCCTTCATTCCATGATGAGGACACTGCCAG(+CAGTGT)GATCCAGGGGTAAAGCACTGAGCTCAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605223020_ckv3 GAGCTCTGCCTGAGCTCAGTGCTTTACCCCTG(+CAGTGT)GATCCTGGCAGTGTCCTCATCATGGAATGAA NGG 10 8 17 8 24 0 100 5 10
    240605223019_hecx CTCCACACAAACTGAGACATATATAAGAAGAG(+CAGTGT)GATCCTGAATTGAGAAATTGCTTCCATCCAG NGG 10 8 17 8 24 0 100 5 10
    240605223017_v03x GCGTCCTGGATGGAAGCAATTTCTCAATTCAG(+CAGTGT)GATCCTCTTCTTATATATGTCTCAGTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605223015_qarq ATCTGGCAGGTCTCATCTCCATTGGGTGGCAG(+CAGTGT)GATCCTGGGGAAGGAACCAACAGCTCCTCCG NGG 10 8 17 8 24 0 100 5 10
    240605223012_4sej GCCCGCGGAGGAGCTGTTGGTTCCTTCCCCAG(+CAGTGT)GATCCTGCCACCCAATGGAGATGAGACCTGC NGG 10 8 17 8 24 0 100 5 10