Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223122_p5uh CTTTTATTGAAGCAATAGTAGGTCTGGCTGAG(+CAGTGT)GATCCATCCATAAATACCCTCCACTATTTCT NGG 10 8 17 8 24 0 100 5 10
    240605223120_4fv3 TTACAAGAAATAGTGGAGGGTATTTATGGATG(+CAGTGT)GATCCTCAGCCAGACCTACTATTGCTTCAAT NGG 10 8 17 8 24 0 100 5 10
    240605223119_jjt4 AGGCAAGAGCATGGTTGTTCGCTTAACAGGAG(+CAGTGT)GATCCTAACACTGTGCAGTGCAAAGAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605223117_b31v TAGCAGCCTTTCTTTGCACTGCACAGTGTTAG(+CAGTGT)GATCCTCCTGTTAAGCGAACAACCATGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605223115_162n CTGTCTCAGAAGAGCATCTGAACTTGTCCTCG(+CAGTGT)GTACCAAGGAATCTCTCCAACAACTGGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605223113_z9k4 AATCAAGACCCAGTTGTTGGAGAGATTCCTTG(+CAGTGT)GTACCGAGGACAAGTTCAGATGCTCTTCTGA NGG 10 8 17 8 24 0 100 5 10
    240605223112_pb46 TGAGCAATTAGAAAATAAGGCTGAATACAGAG(+CAGTGT)GTACCTTTAAAATTACAAAGAGACTTAACAT NGG 10 8 17 8 24 0 100 5 10
    240605223111_vzlg GCCTTATGTTAAGTCTCTTTGTAATTTTAAAG(+CAGTGT)GTACCTCTGTATTCAGCCTTATTTTCTAATT NGG 10 8 17 8 24 0 100 5 10
    240605223109_i3c0 GCATCTGAAAGGCATTACCTAGCAGGAATGAG(+CAGTGT)GTACCCTGTGATCACTATTCCTGGTTATCAA NGG 10 8 17 8 24 0 100 5 10
    240605223108_300n TCAAGTTGATAACCAGGAATAGTGATCACAGG(+CAGTGT)GTACCTCATTCCTGCTAGGTAATGCCTTTCA NGG 10 8 17 8 24 0 100 5 10