Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223214_q9hj TGAACTACAAGAACGGTTTGCTGTTTCTGCTG(+CAGTGT)GATCCTGTCTTCCTTCCTCAACAAGCCACAA NGG 10 8 17 8 24 0 100 5 10
    240605223212_86io TTTTATTGTGGCTTGTTGAGGAAGGAAGACAG(+CAGTGT)GATCCAGCAGAAACAGCAAACCGTTCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605223211_ezj3 TTGGGGAGCCTCAGGACATGGCATGCTTTGTG(+CAGTGT)GATCCCCATGATAGTCTACAAAAGCACCCCG NGG 10 8 17 8 24 0 100 5 10
    240605223209_t8ek AGGGGCGGGGTGCTTTTGTAGACTATCATGGG(+CAGTGT)GATCCACAAAGCATGCCATGTCCTGAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605223207_t2z9 GGCCAGGAAAGGGTTAATGAGAGCCAAGGTGG(+CAGTGT)GTACCTGGAACCATGGCTGGAGGCCTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605223205_0ofc TGCTTTCCCCAGGCCTCCAGCCATGGTTCCAG(+CAGTGT)GTACCCACCTTGGCTCTCATTAACCCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605223203_5tjh TGGCCTCCCAACCAAGGGAGCCATCATGGTGG(+CAGTGT)GTACCAAGAAAGGCAAAGCACTTGGGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605223202_6uov AAAAAAAAGCCCCAAGTGCTTTGCCTTTCTTG(+CAGTGT)GTACCCACCATGATGGCTCCCTTGGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605223200_bzdc TGTGGCCTCAGGCCTCAGTTCTTGGCCAACTG(+CAGTGT)GTACCTGAATGTCTTGTTCTCATTTAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605223159_avz1 AAGCTGCCCCTAAATGAGAACAAGACATTCAG(+CAGTGT)GTACCAGTTGGCCAAGAACTGAGGCCTGAGG NGG 10 8 17 8 24 0 100 5 10