Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223307_m822 GGCAAGTCTGCCTAGCTGTGCTCTGAGGACAG(+CAGTGT)GATCCTAACTGACAGCCTACACTTTGTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605223305_srl9 TGTTAATCTACAAAGTGTAGGCTGTCAGTTAG(+CAGTGT)GATCCTGTCCTCAGAGCACAGCTAGGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240605223304_ny4k TCCTCAAAGCTAAAAACGGCTGCCTGTGTGTG(+CAGTGT)GATCCTGCTCTCTCTTGAGGTCGTTACCTCA NGG 10 8 17 8 24 0 100 5 10
    240605223302_8ts0 CAGTCTGAGGTAACGACCTCAAGAGAGAGCAG(+CAGTGT)GATCCACACACAGGCAGCCGTTTTTAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605223301_ee3i CATTTCCTCTGAGAACCGCTGAAGATTAGTAG(+CAGTGT)GATCCCCACATCTCCCAGTTAAAGCCCAGTT NGG 10 8 17 8 24 0 100 5 10
    240605223259_zs7k GGGAGAACTGGGCTTTAACTGGGAGATGTGGG(+CAGTGT)GATCCTACTAATCTTCAGCGGTTCTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605223257_ech3 GGGCGCTCTCCATGGAACACATGCTCCGGCCG(+CAGTGT)GATCCGCCATCCCTGAGGCTTACGACTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605223255_97q8 AAGAAAGCAAGTCGTAAGCCTCAGGGATGGCG(+CAGTGT)GATCCGGCCGGAGCATGTGTTCCATGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605223252_k2jv CTAGTGTGACTGAGGGCCTAGGTTCAGCCCTG(+CAGTGT)GTACCAACCCAAAAGGAAAAAAAAAAAATCA NGG 10 8 17 8 24 0 100 5 10
    240605223251_yv3q GCTCATGATTTTTTTTTTTTCCTTTTGGGTTG(+CAGTGT)GTACCAGGGCTGAACCTAGGCCCTCAGTCAC NGG 10 8 17 8 24 0 100 5 10