Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223358_5yhp GACAAGAAAAACAACTGTGATGTGGGCAGCAG(+CAGTGT)GTACCTGGGGGTGGAGGGTCTTATTGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605223355_qtx7 CCTGGAGAGGCAATAAGACCCTCCACCCCCAG(+CAGTGT)GTACCTGCTGCCCACATCACAGTTGTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605223354_bw9t CAGGCCAATTATCCTCGGGAAGGAGGGTAAAG(+CAGTGT)GATCCCTCACTATCTTTTGAATTTGGACTGA NGG 10 8 17 8 24 0 100 5 10
    240605223352_vrw2 CTAGATCAGTCCAAATTCAAAAGATAGTGAGG(+CAGTGT)GATCCTTTACCCTCCTTCCCGAGGATAATTG NGG 10 8 17 8 24 0 100 5 10
    240605223350_v64s GAAGCAGGAGAGGAGAGCAAGGAAGTTGACAG(+CAGTGT)GTACCTTTAACTGTCCACTTGACACAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240605223349_yk0f GACTCTAGGCTGTGTCAAGTGGACAGTTAAAG(+CAGTGT)GTACCTGTCAACTTCCTTGCTCTCCTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605223347_o23i GGGACAGTCAGTGGAAAGCTGTTGTACAATAG(+CAGTGT)GATCCAGTCAGCAGGAAGCTAATCAAACAAC NGG 10 8 17 8 24 0 100 5 10
    240605223346_4c7g GCAATGTTGTTTGATTAGCTTCCTGCTGACTG(+CAGTGT)GATCCTATTGTACAACAGCTTTCCACTGACT NGG 10 8 17 8 24 0 100 5 10
    240605223344_mhb3 GTGTGTCCTAGAGAAAGCACAGGAGCCACAGG(+CAGTGT)GTACCCAGGCTGAGACTGAGAGGTGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605223342_brxb TCACCTTCCTTCACCTCTCAGTCTCAGCCTGG(+CAGTGT)GTACCCTGTGGCTCCTGTGCTTTCTCTAGGA NGG 10 8 17 8 24 0 100 5 10