Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223502_97ly GGGCCCTAGGTGGTCAGTCACCTCAGGGCTGG(+CAGTGT)GTACCTGCTAAGGGAGACCCAGGTGGTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605223500_6y4n CCTACGTGAACCACCTGGGTCTCCCTTAGCAG(+CAGTGT)GTACCCAGCCCTGAGGTGACTGACCACCTAG NGG 10 8 17 8 24 0 100 5 10
    240605223459_2zhn GGCGTCCCCGTCTGCTGCAGAGGGGACACTCG(+CAGTGT)GATCCAGCCTGTGGCCACCCCGGCAATAGAC NGG 10 8 17 8 24 0 100 5 10
    240605223458_az9n GTGGTGTCTATTGCCGGGGTGGCCACAGGCTG(+CAGTGT)GATCCGAGTGTCCCCTCTGCAGCAGACGGGG NGG 10 8 17 8 24 0 100 5 10
    240605223455_2nu2 TTGCCTGCAATTCCTGTGGTGTCCACCAGGGG(+CAGTGT)GATCCTAGCACTGTGCCTGCTACAGAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605223454_qxdf CTGAGGCCCTTCTGTAGCAGGCACAGTGCTAG(+CAGTGT)GATCCCCCTGGTGGACACCACAGGAATTGCA NGG 10 8 17 8 24 0 100 5 10
    240605223453_7fst GCTGGCCCCACTCCCTGAGTCTGGCCAAGTTG(+CAGTGT)GATCCTGCTGGTCCTGTTACCAGTTACCTAC NGG 10 8 17 8 24 0 100 5 10
    240605223452_hqrb CCACTGTAGGTAACTGGTAACAGGACCAGCAG(+CAGTGT)GATCCAACTTGGCCAGACTCAGGGAGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605223450_6a7r TAAATAATTATTTAAAAGACAGGAAGTAAGCG(+CAGTGT)GTACCGAGCAATGAAACTGAAGGTACAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605223449_i5oh GCCCAGTGCTGTACCTTCAGTTTCATTGCTCG(+CAGTGT)GTACCGCTTACTTCCTGTCTTTTAAATAATT NGG 10 8 17 8 24 0 100 5 10