Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223554_smq5 GTCTGCAGTGGGCAGGGTGCTGGAGGAGAAGG(+CAGTGT)GATCCAGGCAGACCCCACTCCAACTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240605223552_87fx GTATGTGGTTCAGTTGGAGTGGGGTCTGCCTG(+CAGTGT)GATCCCTTCTCCTCCAGCACCCTGCCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605223550_orz1 GCATTTTTTTTCCATAGGTAACTAACACCATG(+CAGTGT)GATCCAACCAGGCAGAAGTTTGGAGCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605223548_w9fu AGCAAAACTTGCTCCAAACTTCTGCCTGGTTG(+CAGTGT)GATCCATGGTGTTAGTTACCTATGGAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605223547_g1yu AGACCCTATACAAGCAGCATCGCTGTTGCCAG(+CAGTGT)GATCCTCTTCCTAATGAAGGGTCGCAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605223545_o07v ACAGCGTCTCTGCGACCCTTCATTAGGAAGAG(+CAGTGT)GATCCTGGCAACAGCGATGCTGCTTGTATAG NGG 10 8 17 8 24 0 100 5 10
    240605223543_u9ra CAGTATTACCATTATAAATGCTATCCTCCAGG(+CAGTGT)GATCCGCTGCTCCCACTAGCATAAGCCTTCA NGG 10 8 17 8 24 0 100 5 10
    240605223542_w1as CAAAGTGAAGGCTTATGCTAGTGGGAGCAGCG(+CAGTGT)GATCCCTGGAGGATAGCATTTATAATGGTAA NGG 10 8 17 8 24 0 100 5 10
    240605223540_oigc AGTCCTCTGGAAATTCAGATGAAAGGCGTGAG(+CAGTGT)GTACCAAAAATAATAATTTGTCAATTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240605223539_vk3v TCCACAACTAAATTGACAAATTATTATTTTTG(+CAGTGT)GTACCTCACGCCTTTCATCTGAATTTCCAGA NGG 10 8 17 8 24 0 100 5 10