Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223612_86ye AAGCTCATGTTCTGACTGCAAGGATCATGTTG(+CAGTGT)GTACCTACCCTTTAGGAAACTTGCCAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605223610_r683 AATTTCTGACTGGCAAGTTTCCTAAAGGGTAG(+CAGTGT)GTACCAACATGATCCTTGCAGTCAGAACATG NGG 10 8 17 8 24 0 100 5 10
    240605223608_t4bl CTTGGGTCTAGGGTGAATTCATCTTCTGCTAG(+CAGTGT)GTACCCATGGCCTGCAAGTAGATGCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605223605_c4eg AGCCCTGCCTGGCATCTACTTGCAGGCCATGG(+CAGTGT)GTACCTAGCAGAAGATGAATTCACCCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605223604_s6jc ATCTGAAAATGTAATGGCTGCACTCATTCATG(+CAGTGT)GATCCATCTGTCACCCTTCAGACATTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240605223602_1ts1 GAGTGTCTAAAATGTCTGAAGGGTGACAGATG(+CAGTGT)GATCCATGAATGAGTGCAGCCATTACATTTT NGG 10 8 17 8 24 0 100 5 10
    240605223600_qz7p ACATGTAAATTAATATTTAGAGTGAAGGAATG(+CAGTGT)GATCCAATTGATGAAGGAAGATGAGGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605223558_z6e1 TGATAGGCTTCCTCATCTTCCTTCATCAATTG(+CAGTGT)GATCCATTCCTTCACTCTAAATATTAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605223557_ojvd ACCGTTTCGCTAATAGTTACTGCCAGCAGCTG(+CAGTGT)GATCCTGCATACAAAACACTTGTTCATGTTT NGG 10 8 17 8 24 0 100 5 10
    240605223555_l4ak AAGGAAAACATGAACAAGTGTTTTGTATGCAG(+CAGTGT)GATCCAGCTGCTGGCAGTAACTATTAGCGAA NGG 10 8 17 8 24 0 100 5 10