Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223518_7xld ATCCCCGATCCTAGCCCTGTGTCCTGACCATG(+CAGTGT)GTACCACTGCACCCCACACTTGAGCCACAGG NGG 10 8 17 8 24 0 100 5 10
    240605223517_12j7 GGGATCCTGTGGCTCAAGTGTGGGGTGCAGTG(+CAGTGT)GTACCATGGTCAGGACACAGGGCTAGGATCG NGG 10 8 17 8 24 0 100 5 10
    240605223514_2hud CTTCAAGATTGGATTCTGAGCTTAGACCTGGG(+CAGTGT)GTACCCCGCTCCACCTCTCAGTCTTTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605223512_olm1 AGGTCCCCCTAAAGACTGAGAGGTGGAGCGGG(+CAGTGT)GTACCCCAGGTCTAAGCTCAGAATCCAATCT NGG 10 8 17 8 24 0 100 5 10
    240605223510_58ks TAATCCTAGCACTCAGGAGGTGAGGCAGGTGG(+CAGTGT)GTACCTGAGTCTGAGGCTAGATTGGTTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605223509_6fsn CTGGAACTAAACCAATCTAGCCTCAGACTCAG(+CAGTGT)GTACCCACCTGCCTCACCTCCTGAGTGCTAG NGG 10 8 17 8 24 0 100 5 10
    240605223507_apos GGCCTCCCATGGGTTTGGAGGATTTGAACTTG(+CAGTGT)GATCCTTACACCGTTGTGGCAAGCACTTTGC NGG 10 8 17 8 24 0 100 5 10
    240605223506_n3eo TCAGGGCAAAGTGCTTGCCACAACGGTGTAAG(+CAGTGT)GATCCAAGTTCAAATCCTCCAAACCCATGGG NGG 10 8 17 8 24 0 100 5 10
    240605223505_6ojp CCTAAATCAAAGGCTGTCAGCTGCTGGGCAAG(+CAGTGT)GATCCTACAAGGCAGGGACTGGGCCCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605223504_ftbp AGGTGACACAGGGCCCAGTCCCTGCCTTGTAG(+CAGTGT)GATCCTTGCCCAGCAGCTGACAGCCTTTGAT NGG 10 8 17 8 24 0 100 5 10