Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223447_kei3 TGGGCACCTCTGCCCTGCCTGTCTGCTGCCCG(+CAGTGT)GATCCTCCTCACTTAGCACCTAGGGGTGTCA NGG 10 8 17 8 24 0 100 5 10
    240605223445_zb9y GACCCTGACACCCCTAGGTGCTAAGTGAGGAG(+CAGTGT)GATCCGGGCAGCAGACAGGCAGGGCAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605223444_9cvy CTGCTATACCCTGGGAAGGACAACCATCAGTG(+CAGTGT)GTACCTTAGGGGCCTGAGGTTCCAGGGGATC NGG 10 8 17 8 24 0 100 5 10
    240605223442_11pg CTTTAGATCCCCTGGAACCTCAGGCCCCTAAG(+CAGTGT)GTACCACTGATGGTTGTCCTTCCCAGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605223441_yyv4 CACATGCGTGGTCAGTGTCAGTTACCAACTCG(+CAGTGT)GTACCATTCAAAGAGAAAAGATGGATTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605223439_dlxt TGGGAAACAAATCCATCTTTTCTCTTTGAATG(+CAGTGT)GTACCGAGTTGGTAACTGACACTGACCACGC NGG 10 8 17 8 24 0 100 5 10
    240605223437_51sf CCAGCTCCCTCACAGGGGGCTGGGGGGAGGGG(+CAGTGT)GATCCTCAACAAGGACTCCACCCTCACCATG NGG 10 8 17 8 24 0 100 5 10
    240605223436_rvuz GTGGGCATGGTGAGGGTGGAGTCCTTGTTGAG(+CAGTGT)GATCCCCCTCCCCCCAGCCCCCTGTGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605223434_zola GGGCGCGTTTGTTCCTCACCCACGCGCGTGGG(+CAGTGT)GATCCCCTCGCCACAGGGAAGCACAAGAACC NGG 10 8 17 8 24 0 100 5 10
    240605223432_gcxm GAGGGGGTTCTTGTGCTTCCCTGTGGCGAGGG(+CAGTGT)GATCCCCACGCGCGTGGGTGAGGAACAAACG NGG 10 8 17 8 24 0 100 5 10