Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223341_4o4i GGTAGACTCACACAGGGGGCAGTCTGAGATAG(+CAGTGT)GATCCTGCCCCCCCCCCCCCCGCCATGCTAG NGG 10 8 17 8 24 0 100 5 10
    240605223339_0dcs AGGGTCTAGCATGGCGGGGGGGGGGGGGGCAG(+CAGTGT)GATCCTATCTCAGACTGCCCCCTGTGTGAGT NGG 10 8 17 8 24 0 100 5 10
    240605223338_x1l4 TTCTGGATAGGACCCTTGTCATGTAGGAAATG(+CAGTGT)GTACCAATAACCAACCTGTGGGACCTAACGA NGG 10 8 17 8 24 0 100 5 10
    240605223337_bxhc AAACTTCGTTAGGTCCCACAGGTTGGTTATTG(+CAGTGT)GTACCATTTCCTACATGACAAGGGTCCTATC NGG 10 8 17 8 24 0 100 5 10
    240605223335_4a9a TAGGCAAATAGAAGGGTAGCAAAGAGGATATG(+CAGTGT)GTACCCAACACAGAGGAGATCCAAAAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240605223333_pmmd AACATGGCTTTTTTGGATCTCCTCTGTGTTGG(+CAGTGT)GTACCATATCCTCTTTGCTACCCTTCTATTT NGG 10 8 17 8 24 0 100 5 10
    240605223331_jujd GCCAGAAGACAACCTTGGCTCTCATTCCCCAG(+CAGTGT)GTACCATCCAGCTTGGTCTTTAAGATGGGAT NGG 10 8 17 8 24 0 100 5 10
    240605223329_cqpk AAGAGATCCCATCTTAAAGACCAAGCTGGATG(+CAGTGT)GTACCTGGGGAATGAGAGCCAAGGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605223327_noki ATAGCCACTATGCCTCAAGATGTCCATACCAG(+CAGTGT)GATCCAGGTCTGAAACTTCTATCTCCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605223326_v7i5 CTGGAGGCTGGGAGATAGAAGTTTCAGACCTG(+CAGTGT)GATCCTGGTATGGACATCTTGAGGCATAGTG NGG 10 8 17 8 24 0 100 5 10