Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223249_k340 AACCCTCCTCCCACGTCTCCATCAACCTCTGG(+CAGTGT)GATCCAGAGGTAGCTTTGATGCCTGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605223247_iov1 GGAAGCCCTCACAGGCATCAAAGCTACCTCTG(+CAGTGT)GATCCCAGAGGTTGATGGAGACGTGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605223246_m8m1 GTTCGCCCAGGGGCTGGAGAGTTGGCTCAGTG(+CAGTGT)GTACCACATGGTGGCTCACAACCATCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605223244_qnr3 TCTCATTACAGATGGTTGTGAGCCACCATGTG(+CAGTGT)GTACCACTGAGCCAACTCTCCAGCCCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605223242_k9z0 TGGACAAACAGCATGGCTATTTAACATTCTGG(+CAGTGT)GATCCTGCAGAATCCTGCATCTTCCTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605223241_l8qe TTCCCTACTTAGGAAGATGCAGGATTCTGCAG(+CAGTGT)GATCCCAGAATGTTAAATAGCCATGCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605223239_mc4t GGAGGAGGCAGCCTTCAGGCGACAGGCGGGAG(+CAGTGT)GTACCTTCAGTCAGGGGGCATAGGCAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605223238_chr8 GCCTGCCCTCTGCCTATGCCCCCTGACTGAAG(+CAGTGT)GTACCTCCCGCCTGTCGCCTGAAGGCTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605223236_y3kh TGCCACCCCTTCCCTTATCAGGGACAGTATGG(+CAGTGT)GTACCTCAGGTAACCCCAATTTCTGGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605223234_uaf6 AAGTCTCAGGCCAGAAATTGGGGTTACCTGAG(+CAGTGT)GTACCCATACTGTCCCTGATAAGGGAAGGGG NGG 10 8 17 8 24 0 100 5 10