Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223233_d6lp AGTCATCTCTACCACCAGTTCCAGAGAATGTG(+CAGTGT)GTACCCTCTTCTGACCTCTGGGCACAAGGTA NGG 10 8 17 8 24 0 100 5 10
    240605223231_6z4v ATTCATACCTTGTGCCCAGAGGTCAGAAGAGG(+CAGTGT)GTACCACATTCTCTGGAACTGGTGGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605223230_ofoj CTCTGAGCCAGCCCCCACAGCTGAGCTGCTCG(+CAGTGT)GTACCTGACATGGGGTCTTCTGCTGCATTTT NGG 10 8 17 8 24 0 100 5 10
    240605223229_cb58 AAGCCAAAATGCAGCAGAAGACCCCATGTCAG(+CAGTGT)GTACCGAGCAGCTCAGCTGTGGGGGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605223226_2r0g CAGGACAACTCCTGCAGCACTGGGCTGTTGGG(+CAGTGT)GATCCTGGGCACCCCTCTCATGGTGGCGGTG NGG 10 8 17 8 24 0 100 5 10
    240605223224_blel CCACCCACCGCCACCATGAGAGGGGTGCCCAG(+CAGTGT)GATCCCCAACAGCCCAGTGCTGCAGGAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605223222_n0h9 CTAGGCCAGACATGCCCCTGGGAGTGGAAAAG(+CAGTGT)GATCCCTGTTAGCCCAGCAGCAAGGGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605223220_chu0 CCACTTGAGCCCCTTGCTGCTGGGCTAACAGG(+CAGTGT)GATCCTTTTCCACTCCCAGGGGCATGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605223218_11xn AAGGTTTATGTAAGAGCAGGATTGGGGATGAG(+CAGTGT)GATCCCAGGAGGCACAAACTGGGTCAGGTCC NGG 10 8 17 8 24 0 100 5 10
    240605223216_c47r CCATAGGACCTGACCCAGTTTGTGCCTCCTGG(+CAGTGT)GATCCTCATCCCCAATCCTGCTCTTACATAA NGG 10 8 17 8 24 0 100 5 10