Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223627_37yn GTGGTGGTTGGAAGATCTAAATGGGATTCGGG(+CAGTGT)GTACCCCCAAGCATGCACCCCCCTATTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605223624_bume ACTCACTGCAATAGGGGGGTGCATGCTTGGGG(+CAGTGT)GTACCCCGAATCCCATTTAGATCTTCCAACC NGG 10 8 17 8 24 0 100 5 10
    240605223623_8tir AAGGGTTTCCACTTGGGCTGGACCCAGGTTTG(+CAGTGT)GTACCTAGAAGATCCTTCTGCAGACACCGAG NGG 10 8 17 8 24 0 100 5 10
    240605223621_1ys8 AGGTCCTCGGTGTCTGCAGAAGGATCTTCTAG(+CAGTGT)GTACCAAACCTGGGTCCAGCCCAAGTGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605223620_0r2n TTCAAATGAGCTGTATAGAAAGACACCTTGTG(+CAGTGT)GTACCTAAAGTGGAACTGCTTCTTCTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605223619_522p TCACTGAAACAGAAGAAGCAGTTCCACTTTAG(+CAGTGT)GTACCACAAGGTGTCTTTCTATACAGCTCAT NGG 10 8 17 8 24 0 100 5 10
    240605223617_c21g CAGACCCCAGTATATTATTTGATCTTATCTTG(+CAGTGT)GATCCTTTTGAAAAGTTAAGAATATATGAAG NGG 10 8 17 8 24 0 100 5 10
    240605223616_16d0 TTTACCTTCATATATTCTTAACTTTTCAAAAG(+CAGTGT)GATCCAAGATAAGATCAAATAATATACTGGG NGG 10 8 17 8 24 0 100 5 10
    240605223614_0fer TACAGACCTGACTTTCATAGTCAGGAGCTTGG(+CAGTGT)GTACCTGCTTCAGCATCATGGACACTGGGAT NGG 10 8 17 8 24 0 100 5 10
    240605223613_ey90 CTGGAATCCCAGTGTCCATGATGCTGAAGCAG(+CAGTGT)GTACCCAAGCTCCTGACTATGAAAGTCAGGT NGG 10 8 17 8 24 0 100 5 10