Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605223106_imff ATTGTAGTACAACTTTGAATCATCTCAGCAGG(+CAGTGT)GATCCTCGATTGTGAAACTGTCTTGATCATC NGG 10 8 17 8 24 0 100 5 10
    240605223105_gda8 ATTAAGATGATCAAGACAGTTTCACAATCGAG(+CAGTGT)GATCCCTGCTGAGATGATTCAAAGTTGTACT NGG 10 8 17 8 24 0 100 5 10
    240605223103_mwmv CCTAAAGTTCCACAAGGGATGAAGAAGTCCTG(+CAGTGT)GATCCCCCTGTGGTACAGGTGAGAAAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605223100_m8pj GTCTCCTCCTTTTCTCACCTGTACCACAGGGG(+CAGTGT)GATCCAGGACTTCTTCATCCCTTGTGGAACT NGG 10 8 17 8 24 0 100 5 10
    240605223058_uy5o CACCTCTTGACCTCTACACTTCAGGGGCGGGG(+CAGTGT)GTACCCCTCTGAGAATCTAGAAAGGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605223056_8176 GATCTCTGCTTCCTTTCTAGATTCTCAGAGGG(+CAGTGT)GTACCCCCGCCCCTGAAGTGTAGAGGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605223055_imgu TTCCCCCAAACACACATCGCAGAAGAAACATG(+CAGTGT)GTACCATTAGCAAAGCTCTGAAACTTTACAG NGG 10 8 17 8 24 0 100 5 10
    240605223054_7akh AGGATCTGTAAAGTTTCAGAGCTTTGCTAATG(+CAGTGT)GTACCATGTTTCTTCTGCGATGTGTGTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605223051_n0m1 AAGGTAGGCAACAGAGTGGTTTTGGGCACGGG(+CAGTGT)GTACCTCAACTTCGAGGACAGCAAAAACAGC NGG 10 8 17 8 24 0 100 5 10
    240605223050_z236 CTGAAGCTGTTTTTGCTGTCCTCGAAGTTGAG(+CAGTGT)GTACCCCGTGCCCAAAACCACTCTGTTGCCT NGG 10 8 17 8 24 0 100 5 10