Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605222858_i4j6 GGGAGGAAATGAAAATTGGCTCAGAGACGGAG(+CAGTGT)GTACCTCAAAAAAAAAAAAAAAAAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605222857_wdq0 ATTTCTTTTTTTTTTTTTTTTTTTTTTTTGAG(+CAGTGT)GTACCTCCGTCTCTGAGCCAATTTTCATTTC NGG 10 8 17 8 24 0 100 5 10
    240605222856_5ze3 TTCTACATTTGTTCCTGGTGATGAGATTGCTG(+CAGTGT)GATCCTAGATAGTTCCTCGAAGTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240605222854_mcn0 ACTGAGAAGGTCCACTTCGAGGAACTATCTAG(+CAGTGT)GATCCAGCAATCTCATCACCAGGAACAAATG NGG 10 8 17 8 24 0 100 5 10
    240605222853_y00a ATTCTTCAAACATGACTGAGACTGACTCTCAG(+CAGTGT)GATCCTGCTCAGCGCAGGTTCACAGAGGAGC NGG 10 8 17 8 24 0 100 5 10
    240605222851_1e8m TGTCTGCTCCTCTGTGAACCTGCGCTGAGCAG(+CAGTGT)GATCCTGAGAGTCAGTCTCAGTCATGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605222850_1tav CCGGAGATTTAGCAGCTCAGTGTTGGTGTGAG(+CAGTGT)GTACCTGAAACCCCGTCTAATAGGGAGCGGG NGG 10 8 17 8 24 0 100 5 10
    240605222849_0g2b CCCGACCCGCTCCCTATTAGACGGGGTTTCAG(+CAGTGT)GTACCTCACACCAACACTGAGCTGCTAAATC NGG 10 8 17 8 24 0 100 5 10
    240605222847_0ri4 GAGACACCTGCACGCTGGCACACACCCTGAGG(+CAGTGT)GATCCCTGGGACTTCGGCTCCGATGAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605222844_04en CCAAGGTCTCTCATCGGAGCCGAAGTCCCAGG(+CAGTGT)GATCCCTCAGGGTGTGTGCCAGCGTGCAGGT NGG 10 8 17 8 24 0 100 5 10