Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221552_jh5j GAGCTGTAAGTGAGTGTACTCCAGGATACAAG(+CAGTGT)GTACCGATATGTCATGGTCCTCTGACGATCA NGG 10 8 17 8 24 0 100 5 10
    240605221551_mpeg ACACATGATCGTCAGAGGACCATGACATATCG(+CAGTGT)GTACCTTGTATCCTGGAGTACACTCACTTAC NGG 10 8 17 8 24 0 100 5 10
    240605221549_kbc3 TGGATACATCACAGTATGCATGTGAATCGAAG(+CAGTGT)GATCCTTATACATACATAAAGACTTAAATTT NGG 10 8 17 8 24 0 100 5 10
    240605221548_6f9v AATACAAATTTAAGTCTTTATGTATGTATAAG(+CAGTGT)GATCCTTCGATTCACATGCATACTGTGATGT NGG 10 8 17 8 24 0 100 5 10
    240605221546_4imt ACAGCCAGCAAGACTTGGTGGCATTGGGATTG(+CAGTGT)GTACCATTTAACACAGAGAAAGCTCCTTGGA NGG 10 8 17 8 24 0 100 5 10
    240605221545_2brt CCTCCTCCAAGGAGCTTTCTCTGTGTTAAATG(+CAGTGT)GTACCAATCCCAATGCCACCAAGTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605221544_g1zj GAGAACTGTATCTCCCACCTGGAACTGGTGAG(+CAGTGT)GTACCATCAAATCTCCTGGTTCGTAGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605221542_5x6c TCAAGGAGGCCTACGAACCAGGAGATTTGATG(+CAGTGT)GTACCTCACCAGTTCCAGGTGGGAGATACAG NGG 10 8 17 8 24 0 100 5 10
    240605221540_mmuc AAGGGTTACAGATAATAAAGAAATCACAAGTG(+CAGTGT)GTACCTTTCTAAGAATTGCATATTTTTTTAG NGG 10 8 17 8 24 0 100 5 10
    240605221538_k9sl AATTCTAAGAAAGCTGTGAGTTATGCAAGATG(+CAGTGT)GTACCTTGGGGTTTTTGAATCAGGGGGATCA NGG 10 8 17 8 24 0 100 5 10