Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221454_w0eb CCAAATGCAGAGAAAGGAGCTTAGGTCCAAAG(+CAGTGT)GTACCCAGCTATTTGTTTTAAAGAAATAGGA NGG 10 8 17 8 24 0 100 5 10
    240605221452_bnoi TAGTCTAGCAATGTGGGGGTCAGGGATGGGAG(+CAGTGT)GATCCATCGCTGGCTTGCTGCTTGCTAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605221451_defg GAGCTCTGCTAGCAAGCAGCAAGCCAGCGATG(+CAGTGT)GATCCTCCCATCCCTGACCCCCACATTGCTA NGG 10 8 17 8 24 0 100 5 10
    240605221449_9fps GCAATGTAACAGATTCTGGTTTAAATATCTGG(+CAGTGT)GATCCACATATGATCTCTGAGCTCCTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605221448_24xl AGCCCTTTGGAGGAGCTCAGAGATCATATGTG(+CAGTGT)GATCCCAGATATTTAAACCAGAATCTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240605221446_pjue GGTGTCAGTGTTTGGAAGCTGATTATGGGATG(+CAGTGT)GATCCCTGCATATGGCAGTCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605221444_p9uq GGATGGACCATCTAGTGACTGCCATATGCAGG(+CAGTGT)GATCCATCCCATAATCAGCTTCCAAACACTG NGG 10 8 17 8 24 0 100 5 10
    240605221442_gwxj GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605221439_y52f GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605221438_1coz AGTGATGACTCAGATGACGACACTTTATAATG(+CAGTGT)GTACCTGGTTGGTGGACAACTCACACAAACT NGG 10 8 17 8 24 0 100 5 10