Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221417_184l CCCTGAATGCCTTTCTAAAGGCTCAGCGAAAG(+CAGTGT)GATCCTTTATGCCTGGTCACATGTCTTTACC NGG 10 8 17 8 24 0 100 5 10
    240605221416_zdbj CTTGCCATTAACCAGGGCTCTCCTGAAGACAG(+CAGTGT)GTACCACATTTTCATTCAGGCTTGTCATTGA NGG 10 8 17 8 24 0 100 5 10
    240605221414_tj6y GGAGATCAATGACAAGCCTGAATGAAAATGTG(+CAGTGT)GTACCTGTCTTCAGGAGAGCCCTGGTTAATG NGG 10 8 17 8 24 0 100 5 10
    240605221413_js9t CTGTGACGGAACTTCCCGTTAGCCATATTTTG(+CAGTGT)GTACCCTGCCTTTCGAAAAGGACACTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605221410_redk TTGTTTGAGAAGTGTCCTTTTCGAAAGGCAGG(+CAGTGT)GTACCAAAATATGGCTAACGGGAAGTTCCGT NGG 10 8 17 8 24 0 100 5 10
    240605221409_x2ki AGCATTTGTCTTTTACACTTGTACTGTTTCGG(+CAGTGT)GATCCCAAAGATAATCTGTGAGACCTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605221406_fnab GGCAAATTTAAGGTCTCACAGATTATCTTTGG(+CAGTGT)GATCCCGAAACAGTACAAGTGTAAAAGACAA NGG 10 8 17 8 24 0 100 5 10
    240605221405_6kib TTCGCAGCGCCCACAGCCGTGTTTTCTGCTTG(+CAGTGT)GATCCCACCCCCTCCCATCCACGGAACGTGG NGG 10 8 17 8 24 0 100 5 10
    240605221403_ewz4 GCAGCCCACGTTCCGTGGATGGGAGGGGGTGG(+CAGTGT)GATCCAAGCAGAAAACACGGCTGTGGGCGCT NGG 10 8 17 8 24 0 100 5 10
    240605221401_ludu GCTAAAGGGTCACTGTGAAAGGACACTGCAAG(+CAGTGT)GATCCCCAGAGGCAGACACCTACAGTGAGTG NGG 10 8 17 8 24 0 100 5 10