Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221327_hdoj ATCAAAACCCCTTTCTGAGACAACTCCGAAAG(+CAGTGT)GTACCAACAAAGAAAAATCTTCACAGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240605221325_w3ne ACAACAGGATAACCAGAAGGAGTTCAGGTGGG(+CAGTGT)GATCCAATTTTGATGATATCACAGAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240605221324_9vos GATCTCTGGCTTCTGTGATATCATCAAAATTG(+CAGTGT)GATCCCCACCTGAACTCCTTCTGGTTATCCT NGG 10 8 17 8 24 0 100 5 10
    240605221322_40ez AGTTGCCATGTGGGAACTGGGAACTGGAGGTG(+CAGTGT)GATCCTCTAGAAGAGCAGCTAGTGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605221321_55kt AGTGGTTAAGAGCACTAGCTGCTCTTCTAGAG(+CAGTGT)GATCCACCTCCAGTTCCCAGTTCCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240605221319_03fi TCTGTAACAGAATTTTCAGTAGAAAGGCCAAG(+CAGTGT)GATCCTTGATTTGTCAATGGTCACCATAGTG NGG 10 8 17 8 24 0 100 5 10
    240605221317_0fj3 CTTATCACTATGGTGACCATTGACAAATCAAG(+CAGTGT)GATCCTTGGCCTTTCTACTGAAAATTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605221316_cwuu TTACAGATTGCTTAAGGACCTGGGCAGCTGTG(+CAGTGT)GATCCACTGGGCTCCTGGGAATAGATGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605221314_x8nn GAAGTTCTGCATCTATTCCCAGGAGCCCAGTG(+CAGTGT)GATCCACAGCTGCCCAGGTCCTTAAGCAATC NGG 10 8 17 8 24 0 100 5 10
    240605221313_2ttj TAGTGAAGACAACTCTGACCTTGGCATAAAAG(+CAGTGT)GATCCAAGTCAGAATCCCAGCTTGGTCAATT NGG 10 8 17 8 24 0 100 5 10