Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221342_xb0z TTAGAAAAAGCTGGTTTTCCCTTTCCCAATAG(+CAGTGT)GTACCAATTGTGAATAGATTCTCAGTAAGGG NGG 10 8 17 8 24 0 100 5 10
    240605221341_vq05 ATGAGATCTTTTTTTACCCCTTTAACATTCAG(+CAGTGT)GTACCCTAGTAGAGAAAAATAAATAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605221339_3svz TTTTATTTATTTATTTATTTTTCTCTACTAGG(+CAGTGT)GTACCTGAATGTTAAAGGGGTAAAAAAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605221338_cs6m TGAGACCGCCATCCATTCCGTTCCAGGAATGG(+CAGTGT)GTACCATGGCTTCTGGGAAGCAACATCCACG NGG 10 8 17 8 24 0 100 5 10
    240605221336_m34c ATCCCCGTGGATGTTGCTTCCCAGAAGCCATG(+CAGTGT)GTACCCATTCCTGGAACGGAATGGATGGCGG NGG 10 8 17 8 24 0 100 5 10
    240605221335_rmpi TATTTACCTACTTATTAAGGGAGATAATTTTG(+CAGTGT)GTACCCTTGGTTATCAGGTAGTAGAGGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605221332_qxep ATTCTCCCACCTCTACTACCTGATAACCAAGG(+CAGTGT)GTACCAAAATTATCTCCCTTAATAAGTAGGT NGG 10 8 17 8 24 0 100 5 10
    240605221331_gxuz ATTCCCCAGAGGAACATTGAGCAGAGTAAATG(+CAGTGT)GTACCCGTCACTGATTGCAAGCCAATGTATC NGG 10 8 17 8 24 0 100 5 10
    240605221330_t9hi AGACTGATACATTGGCTTGCAATCAGTGACGG(+CAGTGT)GTACCATTTACTCTGCTCAATGTTCCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605221328_n0se GTTAGGCCTACTGTGAAGATTTTTCTTTGTTG(+CAGTGT)GTACCTTTCGGAGTTGTCTCAGAAAGGGGTT NGG 10 8 17 8 24 0 100 5 10