Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221257_q08d GGTTTCAACCGGAAACACTTCCCAAACCGCGG(+CAGTGT)GATCCATGCCAGCTATTCAAACAAGTGATTT NGG 10 8 17 8 24 0 100 5 10
    240605221255_a3f5 CCTATAAATCACTTGTTTGAATAGCTGGCATG(+CAGTGT)GATCCCGCGGTTTGGGAAGTGTTTCCGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605221254_bgo1 TACTTACCCAAAATTTGGTAAGAGGGCACTGG(+CAGTGT)GTACCTTAGCAGCACTCCAACACAGTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605221252_3sq6 ACCACGCTCAACTGTGTTGGAGTGCTGCTAAG(+CAGTGT)GTACCCAGTGCCCTCTTACCAAATTTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605221249_947j CCACATGGCATTTACACTCAGCTCCTCCCTTG(+CAGTGT)GATCCTTCCTGAGTGTGGGCATCCCAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605221248_zk73 AGGAAGTGCTTGGGATGCCCACACTCAGGAAG(+CAGTGT)GATCCAAGGGAGGAGCTGAGTGTAAATGCCA NGG 10 8 17 8 24 0 100 5 10
    240605221247_5bx8 GGCCTCTGACTTCTGTGACACCATCAATATTG(+CAGTGT)GATCCTCACTAGGACTCCTCCCAGCAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605221246_w3uu GATCCAAAGCTGCTGGGAGGAGTCCTAGTGAG(+CAGTGT)GATCCAATATTGATGGTGTCACAGAAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605221244_zm0g TGTCATAGGTGCTCTACCCAATCAGCTCACTG(+CAGTGT)GATCCTCACAACAACCCTGTGACGTGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605221243_g3xy GCTAGGAACTCACGTCACAGGGTTGTTGTGAG(+CAGTGT)GATCCAGTGAGCTGATTGGGTAGAGCACCTA NGG 10 8 17 8 24 0 100 5 10