Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221206_m4fi GGACAAGGTTTTCAGGTATATCTTTAAACAAG(+CAGTGT)GATCCAAGACTCCGTGTCCTACAGGCTATTG NGG 10 8 17 8 24 0 100 5 10
    240605221205_omoa TTTATCAATAGCCTGTAGGACACGGAGTCTTG(+CAGTGT)GATCCTTGTTTAAAGATATACCTGAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240605221203_ldb2 CTGGACCACTTTGGGCTACTGTGTCCCTGAGG(+CAGTGT)GATCCTATCCTAGCTAGGAGCCCTTACAAGT NGG 10 8 17 8 24 0 100 5 10
    240605221202_i1gz CTCTGACTTGTAAGGGCTCCTAGCTAGGATAG(+CAGTGT)GATCCCTCAGGGACACAGTAGCCCAAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605221200_b3rq ATGTGTCCCGGGAAAGCCAGAAGAGAGCTCCG(+CAGTGT)GATCCACTGTAGCTGGAGTCAAAGCAAGATG NGG 10 8 17 8 24 0 100 5 10
    240605221158_rhr9 GCACCCATCTTGCTTTGACTCCAGCTACAGTG(+CAGTGT)GATCCGGAGCTCTCTTCTGGCTTTCCCGGGA NGG 10 8 17 8 24 0 100 5 10
    240605221157_q2eh CAGGGCAGCCATACCAGGACTTTGGGGCTCAG(+CAGTGT)GTACCTTTTGCCTTTGGAGCCCCTTAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605221155_fq6t ATTTATGCACTAAGGGGCTCCAAAGGCAAAAG(+CAGTGT)GTACCTGAGCCCCAAAGTCCTGGTATGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605221154_nw0q ATATTCCCAGCCCTCTTCTTGTTTGAAGGCAG(+CAGTGT)GATCCCACTAAATTGCTCTGGCAGGCCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605221152_u7vn TGAGTTCAAGGCCTGCCAGAGCAATTTAGTGG(+CAGTGT)GATCCTGCCTTCAAACAAGAAGAGGGCTGGG NGG 10 8 17 8 24 0 100 5 10