Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221104_g0c7 GTGAAAGCACTTTGTCACTTACAGTATCTGAG(+CAGTGT)GATCCCCTGCTACACTGATGGGAGGTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605221102_a2id CTCCATCCCCACCTCCCATCAGTGTAGCAGGG(+CAGTGT)GATCCTCAGATACTGTAAGTGACAAAGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605221100_ezit GTATACCTGGGAGAATAAGGCAGAGAGAAACG(+CAGTGT)GATCCCCGAAGAGAAGACTAAAGTAGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240605221058_r5oq TTTAACTTTTCTACTTTAGTCTTCTCTTCGGG(+CAGTGT)GATCCGTTTCTCTCTGCCTTATTCTCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605221056_wbpy CATTTAAACATATACAACTTAACAGATTTTAG(+CAGTGT)GTACCAAAGTGCCAACTTGTTTTTATTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605221055_qet4 TCCTGACCAAATAAAAACAAGTTGGCACTTTG(+CAGTGT)GTACCTAAAATCTGTTAAGTTGTATATGTTT NGG 10 8 17 8 24 0 100 5 10
    240605221053_8ory GTGGGTAGATACTGAACAGAAGCAGACAGAGG(+CAGTGT)GTACCATTCTGTAACGCCCTGTTTATACACC NGG 10 8 17 8 24 0 100 5 10
    240605221051_kbeh CCTTTGGTGTATAAACAGGGCGTTACAGAATG(+CAGTGT)GTACCCTCTGTCTGCTTCTGTTCAGTATCTA NGG 10 8 17 8 24 0 100 5 10
    240605221048_7h7x GATTAAAAACTTTTCTTCTTAGTAACCCTGGG(+CAGTGT)GATCCAAACAAGGGTCCTGAGCTACACACCA NGG 10 8 17 8 24 0 100 5 10
    240605221046_rva4 TGGGCTGGTGTGTAGCTCAGGACCCTTGTTTG(+CAGTGT)GATCCCCAGGGTTACTAAGAAGAAAAGTTTT NGG 10 8 17 8 24 0 100 5 10