Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221135_vgn4 CTATTCTCCTTCCAGCCAGCCAGAAATCCTCG(+CAGTGT)GTACCTGCACCAGTGACCCAGCAGTCGCACA NGG 10 8 17 8 24 0 100 5 10
    240605221134_uhmp CTCACTGTGCGACTGCTGGGTCACTGGTGCAG(+CAGTGT)GTACCGAGGATTTCTGGCTGGCTGGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605221132_d951 AACATTCCAGTTTCCCACAGTCTTATCTTTGG(+CAGTGT)GTACCGTTTACAAACTCATGAGGTTGACCTC NGG 10 8 17 8 24 0 100 5 10
    240605221131_hpjd TTACAGAGGTCAACCTCATGAGTTTGTAAACG(+CAGTGT)GTACCCAAAGATAAGACTGTGGGAAACTGGA NGG 10 8 17 8 24 0 100 5 10
    240605221128_49ck CAGAAAGATGGAAACGATCAGGGACAGCCCAG(+CAGTGT)GATCCCTGATGAGAACTCATGATTTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240605221126_yxbr AATGGCGCAAAAAATCATGAGTTCTCATCAGG(+CAGTGT)GATCCTGGGCTGTCCCTGATCGTTTCCATCT NGG 10 8 17 8 24 0 100 5 10
    240605221125_0hny CCCTAGTTTCTGCCCTCCACACATCATCAGTG(+CAGTGT)GTACCTTAAGTCTTATTCCTGCCAAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605221124_6jts GATCTTTCCTTTTGGCAGGAATAAGACTTAAG(+CAGTGT)GTACCACTGATGATGTGTGGAGGGCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605221122_4iha CTGTTGCCTGTGAAGGTCAGAAAAGGGCAGTG(+CAGTGT)GATCCCATGAGGCTGGATTTATAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605221120_nm02 GCTCACAACCATCTATAAATCCAGCCTCATGG(+CAGTGT)GATCCACTGCCCTTTTCTGACCTTCACAGGC NGG 10 8 17 8 24 0 100 5 10