Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221359_y3do GAAGTCACTCACTGTAGGTGTCTGCCTCTGGG(+CAGTGT)GATCCTTGCAGTGTCCTTTCACAGTGACCCT NGG 10 8 17 8 24 0 100 5 10
    240605221357_hqys GGATGGGGAGATGTGCAGGGTGAGGGCAATTG(+CAGTGT)GTACCACAATGCCAAGGTCATGAAGCTAGGA NGG 10 8 17 8 24 0 100 5 10
    240605221356_wwy6 CTCTGTCCTAGCTTCATGACCTTGGCATTGTG(+CAGTGT)GTACCAATTGCCCTCACCCTGCACATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605221354_3nh3 ACATGGCTGTCTCTCAGCAGCCATGACTGTAG(+CAGTGT)GTACCTAGGTTTCATACAGCTCCATGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240605221352_nwwl CTTATGCCTACATGGAGCTGTATGAAACCTAG(+CAGTGT)GTACCTACAGTCATGGCTGCTGAGAGACAGC NGG 10 8 17 8 24 0 100 5 10
    240605221350_9bq8 ACTGAGACCTGGACCTGATGCTTGAAAGGCGG(+CAGTGT)GTACCTTATTGACTGAGCTATGTCAACAGTT NGG 10 8 17 8 24 0 100 5 10
    240605221349_zb0r TCTTGAACTGTTGACATAGCTCAGTCAATAAG(+CAGTGT)GTACCCGCCTTTCAAGCATCAGGTCCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240605221346_jgv2 GAATTCAGTTAGCAACACTACAGGATATCCTG(+CAGTGT)GTACCAATGAATCTGATAATTCTAAATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605221345_wyye CAAGTTTTAATTTAGAATTATCAGATTCATTG(+CAGTGT)GTACCAGGATATCCTGTAGTGTTGCTAACTG NGG 10 8 17 8 24 0 100 5 10
    240605221344_jks4 CTCACCCCTTACTGAGAATCTATTCACAATTG(+CAGTGT)GTACCTATTGGGAAAGGGAAAACCAGCTTTT NGG 10 8 17 8 24 0 100 5 10